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Gangliosides of bovine optic nerve
Author(s) -
Das Salil K.,
McCullough Melba S.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534417
Subject(s) - ganglioside , sialic acid , chemistry , clinical chemistry , biochemistry , lipidology , composition (language) , chromatography , stereochemistry , linguistics , philosophy
Gangliosides from bovine optic nerve were analyzed. The optic nerve contained 129, 98, 97, 80, 31 and 12 μg of GM 1 , GD 1a , GD 1b , GT 1b , GD 3 and GQ 1 gangliosides, respectively, per g of tissue wet wt. These 6 gangliosides altogether contributed 97% of the total sialic acid. GM 3 and GM 2 gangliosides contributed the remaining 3% of total sialic acid. Stearoyl (18∶0) was the predominant acyl group (61–76%) in all gangliosides. There was a marked variation in acyl group composition between GT 1 and most of the other major gangliosides except GD 3 . In comparison to the other gangliosides, GT 1 contained a lower proportion of the stearoyl group and a higher proportion of the oleoyl, nervonoyl and the long chain acyl groups. Both GT 1 and GD 3 gangliosides contained proportionately higher levels of long chain acyl groups (20∶0→24∶0) than did other gangliosides. GD 3 gangliosides showed 2 bands on thin layer chromatography, and the upper band was more distinct than the lower band.