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Quantitative determination of isomeric glycerides, free fatty acids and triglycerides by thin layer chromatography‐flame ionization detector system
Author(s) -
Tanaka Masamichi,
Itoh Toshihiro,
Kaneko Hiroshi
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534379
Subject(s) - monoglyceride , diglyceride , glyceride , chemistry , boric acid , chromatography , flame ionization detector , acetone , triglyceride , chloroform , fatty acid , organic chemistry , gas chromatography , biochemistry , cholesterol
Partial glyceride mixtures, which include 1‐monoglyceride, 2‐monoglyceride, free fatty acid, 1,2‐diglyceride, 1,3‐diglyceride and triglyceride, could be separated from each other on a 3% boric‐acid‐impregnated Chromarod S‐II (silica gel sintered quartz rod) with either chloroform/acetone (96∶4, v/v) or chloroform/acetone/acetic acid (100∶1∶1, v/v) as the developing solvent mixtures. The components separated on the boric‐acid‐impregnated rod were automatically quantitated in a hydrogen flame ionization detector (Iatroscan). The relative responses of 1,2‐diglyceride, 1,3‐diglyceride, free fatty acid and triglyceride were slightly lower than theoretical responses based on weight percentage, whereas 1‐monoglyceride and 2‐monoglyceride showed slightly higher responses. These responses were converged within a maximal error of 5–10% (SD). Boric‐acid‐impregnated rods could be used repeatedly, ca. 5 times without any reconditioning procedure.