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Lecithin‐cholesterol acyltransferase: Inhibition by local anesthetics in plasma from man and experimental animals in vitro
Author(s) -
Bell Frank P.,
Hubert Edna V.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534370
Subject(s) - dibucaine , tetracaine , chemistry , benzocaine , lecithin , in vitro , procaine , sterol o acyltransferase , lecithin—cholesterol acyltransferase , cholesterol , acyltransferase , biochemistry , pharmacology , lidocaine , apolipoprotein b , enzyme , anesthesia , biology , lipoprotein , medicine
Lecithin‐cholesterol acyltransferase (LCAT, EC 2.3.1.43) was assayed in vitro in plasma from normal man, rat, rabbit and dog by following the formation of 14 C‐cholesteryl esters subsequent to labeling the plasma with 14 C‐cholesterol in vitro. In all species examined, various local anesthetics were found to inhibit LCAT when studied over the concentration range of 1 to 5 mM. The order of inhibition was dibucaine>benzocaine>tetracaine>lidocaine>procaine. Since LCAT activity represents the combined effect of a deacylation step and an esterification step, inhibition of LCAT by local anesthetics could theoretically involve either or both steps.