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A colorimetric assay of pancreatic lipase: Rapid detection of lipase and colipase separated by gel filtration
Author(s) -
Roberts Johnna,
Stella Valentino J.,
Decedue Charles J.
Publication year - 1985
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534361
Subject(s) - tributyrin , colipase , lipase , chromatography , chemistry , substrate (aquarium) , pancreatic lipase , triacylglycerol lipase , absorbance , biochemistry , enzyme , biology , ecology
A rapid assay for pancreatic lipase (E.C., glycerol‐ester hydrolase 3.1.1.3) is described. The assay is based on the color change of a pH indicator as butyric acid is released from the substrate tributyrin. A mixture made with tributyrin and the water soluble components of the assay is ideally suited for use as a rapid test as, for example, when assaying chromatography fractions. Quantitative data can be obtained by measuring the disappearance of absorbance at 557 nm versus a blank reaction. The assay has been used in the rapid preparation of colipase‐free lipase and colipase.