Premium
The effect of fatty acid chain length and unsaturation on the chromatographic behavior of triglycerides on latroscan chromarods
Author(s) -
Kramer J. K. G.,
Fouchard R. C.,
Farnworth E. R.
Publication year - 1985
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534288
Subject(s) - degree of unsaturation , lipidology , chemistry , clinical chemistry , carbon number , fatty acid , carbon chain , methylene , double bond , chromatography , organic chemistry , biochemistry , alkyl
The chromatographic behavior of lipid classes on Chromarods with common developing solvents used for the Iatroscan system was influenced by the esterified fatty acids present in the lipid class. The R f value of the lipid class increased with increasing chain length and increasing unsaturation of the fatty acids; the increase in R f value per methylene carbon was about twice that per double bond. The effect was evident in both triglycerides and phosphatidylcholines. This partial separation of molecular species within a lipid class caused widening of the lipid class band on the Chromarods and can result in poorer resolution, particularly in biological samples.