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Characterization of gangliosides of porcine erythrocyte membranes: Occurrence of ganglioside G D3 as major ganglioside
Author(s) -
Suzuki Yasuo,
Suzuki Naoto,
Michi Hiroyasu,
Matsumoto Makoto
Publication year - 1985
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534284
Subject(s) - ganglioside , clinical chemistry , lipidology , erythrocyte membrane , chemistry , membrane , biochemistry , chromatography
Four major ganglioside species were isolated from porcine erythrocyte membranes by DEAE‐Sephadex and Iatrobeads column chromatography. Treatment of the lipids with graded neuraminidase and β‐galactosidase, gas chromatographic analysis of their carboyhydrates, sphingosine bases and molecular species of sialic acid revealed that the structure of these gangliosides were G M3 (NeuAc), G M3 (NeuGc), G D3 (NeuAc) and G D3 (NeuGc), each of which was 16±2 μg, 304±42 μg, 30±3 μg and 240±26 μg, respectively, per gram of the dry erythrocyte stroma. The amount of G M3 and G D3 accounted for more than 95% of total gangliosides of the erythrocytes. Porcine erythrocytes may provide a good source for large scale preparation of ganglioside G D3 which recently was identified as a human melanoma‐associated antigen.