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Surface viscosities of phospholipids alone and with cholesterol in monolayers at the air‐water interface
Author(s) -
Evans R. W.,
Williams M. A.,
Tinoco J.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534225
Subject(s) - monolayer , phosphatidylcholine , phospholipid , chemistry , surface pressure , viscosity , lysophosphatidylcholine , phosphatidic acid , pulmonary surfactant , analytical chemistry (journal) , chromatography , materials science , membrane , biochemistry , physics , mechanics , composite material
Surface viscosities in lipid monolayers at the air‐water interface were measured by the oscillating pendulum method. The logarithms of successive oscillations decreased linearly with time. Surface viscosity is reported here in terms of the rate constant, k, for decay of oscillation. Viscosities were measured as a function of surface pressure at 22±2 C. Lipids investigated included cholesterol, 1‐palmitoyl‐2‐arachidonoyl phosphatidylcholine (PC), 4 other unsaturated PC, 1‐palmitoyl‐2‐stearoyl PC, 1,2‐distearoyl PC, 1‐palmitoyl‐lysophosphatidylcholine, 1‐palmitoyl‐lysophosphatidylserine, tetrapalmitoyl bisphosphatidic acid, and the dipalmitoyl species of PC, phosphatidylethanolamine (PE), phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidic acid. Pressure‐area curves are presented for the saturated phospholipids. Surface viscosities of most of the phospholipids were high and increased with increasing surface pressure. However, surface viscosiries in monolayers of cholesterol, 1‐palmitoyl‐2‐arachidonoyl PC, lysophosphatidylcholine or lysophosphatidylserine were very low and barely detectable under our experimental conditions. One mol % of cholesterol in monolayers of dipalmitoyl PC greatly reduced the surface viscosity of the film and, in mixed films containing 10% or more of cholesterol, surface viscosity was too low to measure. Cholesterol also reduced surface viscosities in monolayers of the other dipalmitoyl phospholipids. It is suggested that cholesterol functions in lung surfactant by reducing the surface viscosity of its highly saturated phospholipid components.