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Lipid analyses of isolated surface membranes of Leishmania Donovani promastigotes
Author(s) -
Wassef Momtaz K.,
Fioretti Thomas B.,
Dwyer Dennis M.
Publication year - 1985
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534216
Subject(s) - glycolipid , phosphatidic acid , phosphatidylglycerol , biochemistry , phosphatidylethanolamine , phosphatidylcholine , chemistry , phospholipid , phosphatidylinositol , phosphatidylserine , cardiolipin , biology , chromatography , membrane , kinase
Constituent lipids of surface membranes (SM) isolated from Leishmania donovani promastigotes were analyzed and compared with those obtained from whole cells and an isolated kinetoplast‐mitochondrion fraction (KM). On a dry weight basis, the total extractable lipids constituted ≈47%, 12% and 24% of the SM, cells and KM, respectively. The total lipids of SM, cells and KM all were composed of ≈70% phospholipids (PL), 20–25% neutral lipids and 5–10% glycolipids. Sterols and diglycerides composed 60% and 30%, respectively, of the various neutral lipid fractions. Several mannose‐ and galactose‐containing glycolipids were fractionated but not identified. The glycolipid fractions from cells and SM had demonstrable antigenic activities with rabbit anti‐SM sera. Striking quantitative differences were apparent between the PL profiles of the 3 cellular components examined. The PL of SM, whole cells and KM, respectively, were composed of: 15%, 51% and 24% phosphatidylcholine; 37%, 13% and 11% phosphatidylethanolamine (PE); 18%, 10% and 14% phosphatidylinositol; 10%, 1% and 4% phosphatidylserine and traces of cardiolipin, phosphatidylglycerol and phosphatidic acid. An unknown PL containing sphingosine, choline and vicinal hydroxyl groups but no free amino moieties made up ≈19% of the PL of SM and whole cells, but it constituted ≈27% of the PL of KM. The PL side chain constituents of whole cells and SM were composed mainly of longchain fatty acids (C18–20). Further, over 50% of the PE of SM was in the alkyl and alK‐1‐enyl ether forms. These SM properties might contribute to the organism's resistance to digestion in the hydrolytic environs of both its insect vector and mammalian hosts.