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The monoenoic fatty acid composition of a marine species of Desulfobulbus grown on lactate
Author(s) -
Taylor James,
Parkes R. John,
Wahle Klaus W. J.,
Calder A. Graham
Publication year - 1985
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534208
Subject(s) - propionate , anaerobic exercise , biochemistry , fatty acid , derivatization , chemistry , biology , composition (language) , food science , chromatography , mass spectrometry , physiology , linguistics , philosophy
The proportions of isomeric monoenoic fatty acids from cell lipids of a marine, anaerobic, sulphatereducing bacterium Desulfobulbus , grown on lactate, were determined by pyrrolidide derivatization and GC‐MS analysis. The predominant fatty acid was C17ŋ1, Δ11 with much smaller proportions of C16∶1, Δ11 and C18∶1, Δ11 and small (<5%) yet significant proportions of C15∶1, Δ11; C16∶1, Δ9; C17∶1, Δ9 and C18∶1, Δ9. If the spectrum of isomers obtained from this organism were synthesized entirely by the anaerobic pathway, it would indicate a wider chain‐length specificity for the ratelimiting β‐hydroxy acyl dehydrase than hitherto has been reported. The high proportion of oddnumbered fatty acids reflected the chain initiation by propionate derived from lactate. The possibility of monoene synthesis by an anaerobic Δ9‐desaturase mechanism is discussed.