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Elongation systems involved in the biosynthesis of erucic acid from oleic acid in developing Brassica juncea seeds
Author(s) -
Agrawal Vishwanath P.,
Stumpf Paul K.
Publication year - 1985
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534203
Subject(s) - elongation , brassica , erucic acid , oleic acid , biochemistry , biosynthesis , chemistry , elongation factor , botany , biology , enzyme , ribosome , rna , materials science , ultimate tensile strength , gene , metallurgy
In the presence of NADPH and malonyl‐CoA, the cell‐free extract of developing Brassica juncea seeds catalyzes the elongation of 14 C‐oleoyl‐CoA to radioactive C20∶1 and C22∶1. The elongation of C18∶1 to C20∶1 shows no marked preference for NADPH or NADH. On the other hand, the elongation of C20∶1 to C22∶1 exhibits a pronounced preference for NADPH. Moreover, the latter elongation system (C20∶1→C22∶1) is more sensitive to inhibition by trichloroacetate and sodium metabisulfite. Inclusion of polyvinylpolypyrrolidone in the grinding buffer for preparation of the cell‐free extract enhances the elongation activity by 5–6 fold. 14 C‐Stearoyl‐CoA, but not 14 C‐palmitoyl‐CoA, also is elongated by this system. The results presented here suggest certain degrees of dissimilarity between the first (C18∶1→C20∶1) and second elongation (C20∶1→C22∶1) systems.