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A convenient spectrophotometric assay for phospholipase D using p ‐nitrophenyl‐phosphocholine as substrate
Author(s) -
Gupta Munishwar N.,
Wold Finn
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534185
Subject(s) - phosphocholine , chromogenic , phospholipase , substrate (aquarium) , phospholipase d , phospholipase c , chemistry , chromatography , enzyme , phospholipase a , lipidology , clinical chemistry , biochemistry , phosphatase , hydrolysis , phospholipase a2 , phospholipid , biology , phosphatidylcholine , membrane , ecology
A simple assay for plant phospholipase D is described. The enzyme hydrolyzes p ‐nitrophenyl‐phosphocholine to p ‐nitrophenyl phosphate, which in the presence of an acid phosphatase generates p ‐nitrophenol (a chromogenic compound). Thus, p ‐nitrophenylphosphocholine can be used for assay of phospholipase D in sources that do not also contain high levels of phospholipase C.