A convenient spectrophotometric assay for phospholipase D using p ‐nitrophenyl‐phosphocholine as substrate | Zendy

Gupta Munishwar N. | Zendy; Wold Finn | Zendy

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A convenient spectrophotometric assay for phospholipase D using p ‐nitrophenyl‐phosphocholine as substrate

Author(s) -

Gupta Munishwar N.,

Wold Finn

Publication year - 1980

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02534185

Subject(s) - phosphocholine , chromogenic , phospholipase , substrate (aquarium) , phospholipase d , phospholipase c , chemistry , chromatography , enzyme , phospholipase a , lipidology , clinical chemistry , biochemistry , phosphatase , hydrolysis , phospholipase a2 , phospholipid , biology , phosphatidylcholine , membrane , ecology

A simple assay for plant phospholipase D is described. The enzyme hydrolyzes p ‐nitrophenyl‐phosphocholine to p ‐nitrophenyl phosphate, which in the presence of an acid phosphatase generates p ‐nitrophenol (a chromogenic compound). Thus, p ‐nitrophenylphosphocholine can be used for assay of phospholipase D in sources that do not also contain high levels of phospholipase C.

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