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Quantitative analyses of hydroxystearate isomers from hydroperoxides by high pressure liquid chromatography of autoxidized and photosensitized‐oxidized fatty esters
Author(s) -
Neff W. E.,
Frankel E. N.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534183
Subject(s) - chemistry , chromatography , high performance liquid chromatography , linolenate , mass spectrometry , fatty alcohol , alcohol , saponification , elution , organic chemistry , fatty acid
A high pressure liquid chromatography (HPLC) method is described for the determination of the isomeric hydroxysterates from hydroperoxides of oxidized fatty esters. The samples are hydrogenated and the mixtures of hydroxystearates are concentrated by partial removal of unoxidized esters and complete removal of polar materials. Isomeric hydroxystearates are then separated on a porous microparticulate adsorption (10 μ) column and elution with 0.25% isopropyl alcohol in n ‐hexane is monitored at 212 nm. The 8‐OH, 9‐OH, 10‐OH, 11‐OH and 16‐OH isomers were completely separated, but the 12‐OH, 13‐OH and 15‐OH were only partly resolved by HPLC. The relative percentages of isomeric hydroxy esters were analyzed quantitatively by a computer integration method. Accuracy of the method was checked with known mixtures of synthetic hydroxystearates. The isomeric hydroperoxide composition of oxidized methyl oleate, linoleate, linolenate and soybean methyl esters determined by HPLC were in good agreement with previous analyses by gas chromatography‐mass spectrometry.