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Product specificity of rice germ lipoxygenase
Author(s) -
Yamamoto A.,
Fujii Y.,
Yasumoto K.,
Mitsuda H.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534108
Subject(s) - lipoxygenase , germ , linoleic acid , chemistry , incubation , lipidology , enzyme , clinical chemistry , biochemistry , chromatography , food science , biology , fatty acid , microbiology and biotechnology
Incubation of linoleic acid with partially purified lipoxygenase from rice germ yielded a ratio of 9‐to 13‐hydroperoxides of linoleic acid of 97∶3 as measured by high performance liquid chromatography. Under similar conditions, hematin gave the 9‐ to 13‐hydroperoxides at a ratio of 51∶49, and soybean lipoxygenase‐a at 9∶91. Infrared spectral analysis revealed cis‐trans configuration to predominate in the reaction products with the rice germ enzyme as was with the soybean enzyme.