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Distribution of hexadecenoic, octadecenoic and octadecadienoic acid isomers in human tissue lipids
Author(s) -
Adlof R. O.,
Emken E. A.
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534049
Subject(s) - structural isomer , adipose tissue , cis–trans isomerism , chemistry , lipidology , fatty acid , chromatography , biochemistry , stereochemistry
The trans 16∶1, 18∶1 and 18∶2 fatty acid composition of various human organ lipids was studied to determine if isomers accumulated in specific tissues. “ Trans ” isomers are defined as those fatty acids containing one or more trans double bonds. Adipose, kidney, brain, heart and liver tissue lipids were analyzed. Gas chromatography with a 100‐SP2560 capillary column was used to characterize the various positional and/or geometrical isomers. The distribution of rans 16∶1 and 18∶1 isomers ranged from 0.3% in the brain to 4.0% in adipose tissue, while trans 18∶2 isomers ranged from 0.0% in the brain to 0.4% in adipose tissue. No trans 18∶3 isomers were detected. Positional isomer ratios for cis 16∶1 (Δ9 vs Δ7) and cis 18∶1 (Δ11 vs Δ9) were also determined. Since these ratios are reproducible from one individual to the next, they might be useful for diagnosis of human metabolic disorders.