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Preparation of radiolabeled tetracosa mono‐ and dienoic acid methyl esters from rat erythrocyte lipids by thin layer chromatography
Author(s) -
Rao G. Ananda,
Kilpatrick Robert L.,
Goheen Steven C.,
Larkin Edward C.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534020
Subject(s) - thin layer chromatography , sphingomyelin , lipidology , chromatography , chemistry , fatty acid methyl ester , fatty acid , clinical chemistry , cholesterol , organic chemistry , biochemistry , catalysis , biodiesel
An easy method of obtaining pure fatty acid methyl esters (FAME) of tetracosa mono‐ and dienoic acids (24∶1, 24∶2) using thin layer chromatography (TLC) is described. The total lipids isolated from rat erythrocytes were treated with methanolic‐NaOH. Sphingomyelin was unaffected by this treatment and was separated from FAME of glycerolipids and cholesterol by TLC. FAME of sphingomyelin were then prepared by acid methanolysis. These esters migrated into 2 distinct bands on TLC. The slow moving band contained FAME of 16∶0, 16∶1, 18∶0, 18∶1, 19∶0 and 20∶0 whereas the fast moving band contained FAME of 22∶0, 23∶0, 24∶0, 24∶1 and 24∶2. After AgNO 3 ‐TLC, the FAME of the fast moving band separated into 3 species; esters of saturated acids, 24∶1 and 24∶2, respectively. With erythrocyte lipids of rats fed a fat‐free diet and injected with 14 C‐18∶1, this method yielded 14 C‐24∶1. From rats injected with 14 C‐18∶2 and maintained on a corn oil diet, 14 C‐24∶2 was obtained.