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Horse erythrocyte gangliosides: Preparation of the major hematoside NeuNG1‐Lac‐Cer
Author(s) -
MagetDana R.,
Michalski J. C.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534019
Subject(s) - lipidology , horse , clinical chemistry , chemistry , biochemistry , biology , paleontology
A simple method for the isolation of hematoside NeuNG1‐Lac‐Cer from horse erythrocytes is described. An aliquot of the crude ganglioside fraction was labeled by tritiated sodium borohydride after mild periodate oxidation. The compounds obtained were used as radioactive tracers in column chromatography. Gangliosides were applied onto a silicic acid column and eluted stepwise by solvents of steadily increasing polarity. The major ganglioside, NeuNG1‐Lac‐Cer, was eluted in a high yield by the solvent mixture chloroform/methanol/water (60∶35∶8, v/v/v).