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Acyltransferases in subcellular fractions of developing seeds of rape ( Brassica napus L.)
Author(s) -
Murphy Denis J.,
Mukherjee Kumar D.
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533995
Subject(s) - phosphatidic acid , lysophosphatidylethanolamine , acyltransferases , chemistry , acyltransferase , biochemistry , lysophosphatidylcholine , microsome , acyl coa , phosphatidylcholine , chromatography , enzyme , phospholipid , biosynthesis , membrane
Acyltransferase activities responsible for the transfer of oleoyl moieties from oleoyl‐CoA to various lipids have been examined in subcellular fractions of developing seeds of rape, Brassica napus L. In the absence of exogenous acyl acceptors, the microsomal and oil body fractions transferred oleoyl moieties mostly to phosphatidylcholines and phosphatidic acids, although there was substantial incorporation of the oleoyl moieties into monoacylglycerols, diacylglycerols and triacylglycerols. The soluble (150,000‐g supernatant) fraction incorporated oleoyl moieties mainly into the neutral lipids (monoacylglycerols and diacylglycerols) and also exhibited a relatively high acyl‐CoA hydrolase activity. In the presence of the exogenous acyl acceptors, lysophosphatidylcholine and lysophosphatidic acid, both microsomal and oil body fractions transferred most of the oleoyl moieties to phosphatidylcholines and phosphatidic acids, respectively. Other lysophospholipids, such as lysophosphatidylethanolamine and lysophosphatidylonositol, were not very effective as acyl acceptors, nor were glycerol‐3‐phosphate, monoacylglycerols or diacylglycerols. In contrast, the soluble fraction showed little or no stimulation of acyltransfer in the presence of exogenous lysophospholipids but was able to utilize, to some extent, exogenous monoacylglycerols and diacylglycerols as acyl acceptors.