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Characterization of feline omentum lipids
Author(s) -
McCluer Robert H.,
Evans James E.,
Williams Marcia,
Griffith Ann L.,
Catsimpoolas Nicholas
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533984
Subject(s) - chromatography , chemistry , lactosylceramide , phosphatidylethanolamine , glycolipid , high performance liquid chromatography , sphingomyelin , phosphatidylcholine , thin layer chromatography , phospholipid , biochemistry , cholesterol , membrane
Feline omental lipid extracts, previously reported to be angiogenic in the cornea of rabbits, were fractionated and the major lipid components characterized. Approximately 97% of the chloroform/methanol extract consisted of triglycerides containing primarily 16∶0, 18∶0, 18∶1 and 18∶2 fatty acids. Trace quantities of free fatty acids, cholesterol, di‐ and monoglycerides were also detected. The phospholipid fraction, obtained by solvent partition and Unisil column chromatography and characterized by high performance liquid chromatography (HPLC)‐mass spectrometry, was found to consist of phosphatidylcholine, sphingomyelin, phosphatidylethanolamine and phosphatidylserine. The neutral glycolipids, isolated by solvent partition and Unisil column chromatography and identified by high performance thin layer chromatography and HPLC of their perbenzoylated derivatives, were found to consist of glucosyl‐ and galactosylceramides, galabiosylceramide, lactosylceramide, globotriaosylceramide and globotetraosylceramide. The complex glycolipid fraction, obtained from Folch upper phase solvent partition, was found to consist primarily of Forssman glycolipid and gangliosides GM 3 and GD 3 . Smaller amounts of GM 1 and other unidentified gangliosides were also present.