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Lipid composition of bacillus megaterium spores and spore membranes
Author(s) -
Nikolopoulou M.,
Vary J. C.
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533968
Subject(s) - bacillus megaterium , spore , lipidology , composition (language) , chemistry , membrane , food science , biochemistry , biology , microbiology and biotechnology , clinical chemistry , bacteria , genetics , linguistics , philosophy
Bacillus megaterium QM B1551 spore lipids were extracted by an improved technique, and the phospholipid and fatty acid compositions were determined. Phospholipids accounted for 65% of the total fatty acids; the neutral lipid fraction contained 15% and the remaining fatty acids were in the interphase, aqueous phase and pellet from the lipid extraction. Each phospholipid had similar fatty acid compositions as did the delipidated pellet. However, the aqueous phase and, to some extent, the interphase had unique fatty acid compositions. Also, fatty acids were found acylated to proteins, which was observed by electrophoresis of delipidated proteins from spores grown in [1‐ 14 C]palmitate. Therefore, spores contain unique non‐phosphatide fatty acid components that can now be analyzed.