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Monohydroperoxides of linoleic acid in endoplasmic lipids of rats exposed to tetrachloromethane
Author(s) -
Frank Hartmut,
Wiegand Manfred,
Strecker Manfred,
Thiel Dietmar
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533967
Subject(s) - microsome , lipidology , clinical chemistry , chemistry , incubation , microsoma , endoplasmic reticulum , linoleic acid , chromatography , lipid peroxidation , biochemistry , fatty acid , antioxidant , in vitro
An accurate method for the quantitative determination of hydroperoxy and hydroxy fatty acids in liver microsomes is presented which involves the use of 18 O‐labeled internal standards. The method is employed for the determination of hydroperoxides in rat liver microsomes after aerobic incubation with Fe 2+ /ADP and in microsomes from animals exposed to 75 mg tetrachloromethane/kg body weight. The amounts found after artificial microsomal “lipid peroxidation” are almost two orders of magnitude larger than those in microsomes from tetrachloromethaneexposed animals.