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Isolation and quantitation of lectins from vegetable oils
Author(s) -
Klurfeld David M.,
Kritchevsky David
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533947
Subject(s) - lectin , sunflower , chemistry , soybean oil , corn oil , food science , chromatography , sunflower oil , vegetable oil , edible oil , clinical chemistry , lipidology , biochemistry , biology , agronomy
The factor(s) responsible for the unexplained atherogenicity of peanut oil remain to be elucidated. To this end, we developed a technique to determine if lectin was present in the oil and to quantitate its concentration. This technique was applied to other vegetable oils including corn, soybean, and sunflower. Crude, unprocessed corn and soybean oils were also analyzed for lectin content. The crude oils contained from 858 to 2983 μg lectin per kg, while the refined oils contained 24 to 55 μg/kg of biologically active lectin. The identities of the isolated lectins were confirmed by electrophoresis on SDS‐polyacrylamide gels. The biological significance of the presence of lectin in these oils remains to be determined.