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Lipid composition of cultured B16 melanoma cell variants with different lung‐colonizing potential
Author(s) -
Calorini L.,
Fallani A.,
Tombaccini D.,
Mugnai G.,
Ruggieri S.
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533944
Subject(s) - lipidology , clinical chemistry , melanoma , cell , biochemistry , composition (language) , biology , phospholipid , polyunsaturated fatty acid , ether , chemistry , cancer research , fatty acid , organic chemistry , linguistics , philosophy , membrane
Lipid components influence several cell surface properties that are critical in different stages of the metastatic process. In this study, we examined whether the different lung‐colonizing potential of B16‐F1 and B16‐F10 melanoma cells could be related to a characteristic lipid profile. The lipid analyses, carried out on the same cell cultures used for the assay of lung‐colonizing potential, revealed characteristics in the lipid composition of both B16‐F1 and B16‐F10 melanoma cells that are common to other systems of malignant cells: a high level of 18∶1 associated with low proportions of polyunsaturated fatty acids in phospholipids, accumulation of ether‐linked lipids and absence of complex gangliosides. The two B16 melanoma variants differed significantly only with respect to ether‐linked lipids, due to a higher level of alkyl‐PC in B16‐F10 than in B16‐F1.