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Trans fatty acids: Positional specificity in brain lecithin
Author(s) -
Karney Rona I.,
Dhopeshwarkar Govind A.
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533911
Subject(s) - chemistry , lecithin , phospholipase a2 , clinical chemistry , glyceride , arachidonic acid , fatty acid , lipidology , biochemistry , enzyme
Fifteen‐day‐old rats were divided into three groups: one group received an intracerebral injection of 5 μ Ci of 9‐ trans , 12‐ trans [1‐ 14 C] octadecadienoic acid; the second group was given 5 μCi of the same compound plus an equal wt of nonradioactive all cis arachidonic acid; the third group was given 5 μCi of 9‐ trans [1‐ 14 C] octadecenoic acid. All animals were sacrificed 8 hr after injection. Glycerophosphocholine (GPC) was isolated and partically deacylated with phospholipase A 2 from Crotalus Adamanteus venom. The results of this study were as follows: 1) after t [1‐ 14 C] 18∶1 injection, there was twice as much radioactivity in the 1‐position as in the 2‐position; 2) when tt [1‐ 14 C] 18∶2 was injected, more than 90% of the total radioactivity was found in the 2‐position; 3) following tt [1‐ 14 C]‐18∶2 +nonradioactive arachidonate injection, ca. 75% of the total radioactivity still remained in the 2‐position; and 4) all of the injected [1‐ 14 C]‐tracers showed evidence of undergoing β‐oxidation to form acetyl‐CoA, which was converted to radioactive palmitate. The possibility is discussed that the observed distribution pattern of the injected radioactive tracers may be attributed to tissue metabolic specificity. Ramifications of the deposition of dietary trans fatty acids in the brain during the developmental stage of the central nervous system are also discussed.