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A specific spectrophotometric determination of long chain nitro compounds and its application to NO 2 ‐initiated lipid autoxidation
Author(s) -
Collard Randle S.,
Pryor William A.
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533901
Subject(s) - autoxidation , chemistry , nitro , amine gas treating , chromatography , organic chemistry , nitro compound , alkyl
A specific spectrophotometric method is described for the determination of nitro‐β‐hydroperoxides produced during the initiation of lipid autoxidation by ppm NO 2 ; this determination provides a measure of the rate of initiation by addition of NO 2 to lipids. The nitro group is reduced to an amine using polymethylhydrosilane (PMHS) and Pd on charcoal, and the amine is then determined spectrophotometrically as a neutral methyl orange salt. Although this method was developed for the determination of micromolar quantities of nitro‐β‐hydroperoxides in lipid samples, it can be used to determine any long chain, nonpolar nitro compound. The method also can be adapted to allow the determination of polar or short chain nitro compounds by using a different amine determination.