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The effects of the hypocholesteremic compound 3β‐(β‐dimethylaminoethoxy)‐androst‐5‐en‐17‐one on the sterol and steryl ester composition of Saccharomyces cerevisiae
Author(s) -
Field Ruth B,
Holmlund Chester E.,
Whittaker Noel F.
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533900
Subject(s) - sterol , lipidology , clinical chemistry , cholesterol , chemistry , composition (language) , biochemistry , organic chemistry , philosophy , linguistics
When yeast was grown in the presence of 10 −4 M 3β‐(β‐dimethylaminoethoxy)‐androst‐5‐en‐17‐one (DMAE‐DHA), the compound 2,3;22,23‐dioxidosqualene (DOS) accumulated. Total free sterol was reduced by about 30%, whereas almost no steryl esters were found. The same drug at lower concentration (3×10 −6 M) caused a slight increase in steryl ester production, and a 24% reduction in free sterol content. The marked accumulation of ergostra‐5,7,22,24(28)‐tetraen‐3β‐ol with 3×10 −6 M DMAE‐DHA indicated that the C 24–28 reductase is especially sensitive to the action of the drug.