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Effect of calcium on absorption of fatty acid by rat jejunum in vitro
Author(s) -
Saunders D. R.,
Sillery J.
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533893
Subject(s) - jejunum , lipidology , clinical chemistry , calcium , in vitro , fatty acid , absorption (acoustics) , chemistry , biochemistry , materials science , organic chemistry , composite material
The effect of Ca ++ on jejunal osmiophilic particles was studied in a recirculating system which was not contaminated with plasma lipoproteins. An isolated, infused segment of rat jejunum was suspended in a bath of liquid paraffin. Transudate, containing osmiophilic particles, appeared like beads of sweat on the serosal surface, and fell to the bottom of the bath. In the range of 25–38 C, 30 C proved to be optimal for histological preservation of villous architecture. Production of transudate, 20 mg/min/g of jejunum, and transport of [ 14 C] oleate proceeded nearly linearly after the first 30 min. Necrosis of mid‐villus and crypt cells became obvious by light microscopy after one hour. Therefore, transudate was collected between the period of 30–60 min. Shadow casting of transudates, produced when saline was infused, revealed that 86±9 (SD) % of osmiophilic particles was <800 Å in diameter; 13±8% was 800–1000 Å; 0.4±0.5% was 1000–2000 Å. Corresponding values were 58±10, 25±5, and 16±5% when 5 mM [ 14 C] oleate+2.5 mM monoolein was infused; 75% of the transported [ 14 C] appeared in triglyceride. Adding 2 mM Ca ++ to the infusion doubled the transport of [ 14 C] triglyceride without increasing particle size further. We conclude that luminal Ca ++ increases the absorption of luminal fatty acid by rat jejunum in vitro.