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Interaction of human plasma high density lipoprotein HDL 2 with synthetic saturated phosphatidylcholines
Author(s) -
Gong Elaine L.,
Nichols Alex V.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533881
Subject(s) - chemistry , chromatography , phosphatidylcholine , phospholipid , ultracentrifuge , redistribution (election) , incubation , lipoprotein , centrifugation , biochemistry , cholesterol , membrane , politics , political science , law
The interaction of human plasma high density lipoprotein HDL 2 (d 1.063–1.125 g/ml) with sonicated dispersions of synthetic saturated phosphatidylcholines, dipalmitoyl‐ (diC 16 PC), dimyristoyl‐ (diC 14 PC), didodecanoyl‐ (diC 12 PC), didecanoyl‐ (diC 10 PC), and dioctanoyl‐ (diC 8 PC) L‐alpha phosphatidylcholine, was investigated. Incubation (4.5 hr, 37 C) of HDL 2 with diC 14 PC, diC 12 PC, diC 10 PC and diC 8 PC followed by gradient gel electrophoresis or preparative ultracentrifugation resulted in a redistribution of apolipoprotein A‐I (apoA‐I). The extent of redistribution depended on the molar ratio of the phospholipid to HDL 2 in the incubation mixture. Redistributed apoA‐I occurred as lipid‐free apoA‐I and/or as complexes of apoA‐I with phosphatidylcholine. Increasing the length of time of ultracentrifugation of the interaction mixtures did not increase the extent of redistribution. No redistribution of apoA‐I was detected following incubation and gradient gel electrophoresis or preparative ultracentrifugation of mixtures of HDL 2 with dispersions of diC 16 PC.