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Separation of triglycerides by chain length and degree of unsaturation on silica HPLC columns
Author(s) -
Plattner R. D.,
PayneWahl Kathleen
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533865
Subject(s) - degree of unsaturation , triolein , chemistry , chromatography , elution , silica gel , diethyl ether , high performance liquid chromatography , solvent , double bond , fractionation , organic chemistry , lipase , enzyme
Triacylglycerols can be separated by both chain length and number of double bonds using micro particulate silica high pressure liquid chromatography columns with isooctane, diethyl ether, and acetic acid solvent mixtures. The separations obtained are the reverse of those observed with μ‐Bondapak C 18 columns (Waters Associates); i.e., longer chain length triglycerides elute from the column earlier than their shorter chain homologs, and saturated triglycerides elute before the more unsaturated ones. Base line separation was obtained between tristearin, triolein, trilinolein, and trilinolenin.