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Studies of serum lecithin‐cholesterol acyl transferase activity in rat: Effect of vitamin E deficiency, oxidized dietary fat, or intravenous administration of ozondies or hydroperoxides
Author(s) -
Takatori Takehiko,
Privett O. S.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533829
Subject(s) - lecithin , chemistry , cholesterol , clinical chemistry , transferase , endocrinology , medicine , vitamin , egg lecithin , enzyme , lipidology , biochemistry , biology
Serum lecithin‐cholesterol acyl transferase activity in the rat was studied in animals raised on diets devoid of vitamin E or containing oxidized fat and in the serum of normal animals after intravenous administration of hydroperoxides or ozonides. Lecithin‐cholesterol acyl transferase activity was suppressed by a vitamin E deficiency but was elevated in the serum of the animals fed diets containing oxidized fat. The intravenous injection of hydroperoxides or ozonides of linoleate into the tail vein of rats caused an immediate depression of serum lecithin‐cholesterol acyl transferase activity. The effect of hydroperoxides was more severe than the ozonides, but, with sublethal doses of these compounds, the activity of the enzyme became normal within 24 hr. Hydroperoxides and ozonides also suppressed the activity of lecithin‐cholesterol transferase of rat serum of normal animals in vitro. The suppression of lecithin‐cholesterol acyl transferase activity by hydroperoxides of ozonides was only partially restored by the addition to the serum of 2‐mercaptoethanol.