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Metabolism of 2,4‐ 3 H‐14α‐methyl‐5α‐ergost‐8‐enol and 2,4‐ 3 H‐5α‐ergosta‐8,14‐dienol in Chlorella ellipsoidea
Author(s) -
Tsai L. B.,
Patterson G. W.,
Cohen C. F.,
Klein P. D.
Publication year - 1974
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533828
Subject(s) - ergosterol , enol , chemistry , chlorella , algae , biochemistry , stereochemistry , botany , biology , catalysis
5α‐Ergosta‐8,14‐dienol and 14α‐methyl‐5α‐ergost‐8‐enol were synthesized chemically from ergosterol and labeled with tritium at the C‐2 and C‐4 positions. Both labeled sterols, when incubated with growing cultures of Chlorella ellipsoidea , were converted to ergost‐5‐enol but not to C‐27 or C‐29 sterols. Chlorella ellipsoidea , thus, has the capability of removing the C‐14 methyl group and converting the 8(9) and 8,14 double bond systems to the 5(6) position. Brassicasterol produced by this organism is not labeled in these experiments, indicating that it is not derived from a saturated side chain precusor.