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Accumulation of ergosta‐8,14‐dien‐3β‐ol by Saccharomyces cerevisiae cultured with an azasterol antimycotic agent
Author(s) -
Hays P. R.,
Parks L. W.,
Pierce H. D.,
Oehlschlager A. C.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533762
Subject(s) - ergosterol , saccharomyces cerevisiae , sterol , clinical chemistry , yeast , chemistry , biochemistry , strain (injury) , biosynthesis , reductase , lipidology , stereochemistry , enzyme , biology , cholesterol , anatomy
15‐Aza‐24‐methylene‐D‐homocholesta‐8,14‐dien‐3β‐ol, an antimycotic agent, at a concentration of 75 ng/ml inhibited ergosterol biosynthesis in Saccharomyces cerevisiae strain 3701 B resulting in the accumulation of an unusual sterol. Experimental data presented indicate that this sterol is ergosta‐8,14‐dien‐3β‐ol. The accumulation of the compound is supportive of current models of biosynthetic pathways for sterols in yeast and is consistent with inhibition by the azasterol of the Δ 14 sterol reductase.