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The effect of protein deficiency on some rat liver lipid metabolic enzymes and CoA
Author(s) -
Gerson T.,
Wong M. N.
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533716
Subject(s) - biochemistry , enzyme , microsome , oleic acid , stearic acid , biology , clinical chemistry , fatty acid , palmitic acid , acyl coa , dehydrogenase , in vivo , chemistry , microbiology and biotechnology , organic chemistry
Abstract Two groups of male Wistar rats were fed normal (i.e., 18%) and protein‐free diets, respectively, for 7 weeks. In vivo incorporation of [1‐ 14 C] acetate into palmitic, stearic, oleic, and arachidonic acids by the liver was reduced in the protein‐deficient rats. In vitro incubation of liver microsomes with labeled palmitate or linoleate revealed no change in the specific activities of chain elongating or desaturating enzymes. Protein deficiency resulted in a decrease in specific activity of short chain acyl‐CoA synthetase and in total CoA, accompanied by the virtual disappearance of acyl‐CoA and an increase in free CoA. Furthermore, there was less microsomal fatty acid synthetase and mitochondrial β‐hydroxybutyrate dehydrogenase activity. These results are discussed in relation to fatty acid synthesis and the changes in liver fatty acid composition.