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Mass fragmentographic determination of docosenoic acid in rapeseed oils
Author(s) -
Blomstrand Rolf,
Svensson Lennart,
Herslöf Bengt
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533671
Subject(s) - erucic acid , rapeseed , chemistry , chromatography , mass spectrometry , gas chromatography , methanol , sodium methoxide , reagent , extraction (chemistry) , gas chromatography–mass spectrometry , organic chemistry , food science
A highly sensitive and accurate reference method for determination of docosenoic acid (mainly erucic acid, 22∶1n−9) in different rapeseed oils is described. A fixed amount of [1‐ 14 C]erucic acid methyl ester (about 1 μg) is added to a fixed amount of oil. After treatment with sodium methoxide/methanol reagent and extraction with hexane, the amount of unlabeled erucic acid is determined from the ratio between the recordings at m/e 320 and m/e 322 obtained after analysis with a combined gas chromatograph‐mass spectrometer equipped with an MID (multiple ion detector). The two ions used correspond to the M‐32 peak in the mass spectrum of unlabeled and [1‐ 14 C]labeled erucic acid methyl ester. The relative standard deviation of the method is about 1.8%. The method was compared with a gas chromatographic method for determination of erucic acid.