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Erucic acid and phospholipids of newborn rat heart cells in culture
Author(s) -
Rogers C. G.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533641
Subject(s) - lipidology , erucic acid , clinical chemistry , chemistry , heart cells , biochemistry , medicine , fatty acid , myocyte
Erucic acid (Δ 13 ‐docosenoic acid), labeled with 14 C in the 1‐or 14‐position, was incorporated into fetal calf serum and fed to beating, neonatal rat myocardial cells in culture. Uptake of the docosenoic acid during the first 6 hr of incubation was 41 nM/hr/mg protein in 7‐day old cells and 29 nM/hr/mg protein in 14‐day old cells. Fifty‐seven percent of the 14 C‐activity was taken up from the medium in 24 hr, of which 77% was in the cells and 23% was unaccounted for. Of the 14 C‐activity taken up, 26% was in extractable lipid, with two‐thirds in neutral lipid and one‐third in phospholipid. Within the neutral lipid fraction, 88% of the 14 C‐activity was present in triglycerides; while in phospholipids, 66% of the 14 C‐activity was in phosphatidylcholine (PC); 14% in phosphatidylethanolamine (PE); 6% in sphingomyelin (SPH) and 1% or less in cardiolipin (DPG). PC had the highest specific activity, followed by SPH and PE. The specific activity of PE was one‐half that of SPH when the 14 C‐erucic acid substrate was labeled at the carboxyl position, but increased to equal that of SPH when the substrate was labeled at the double bond. The fatty acids of PC, PE, and SPH were influenced by erucic acid in the growth medium, but the amounts of each phospholipid were not affected. It is proposed that the altered fatty acid composition associated with incorporation of erucic acid or its metabolites into PC, PE, and SPH may affect integrity and function of heart cell membranes.