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Bis ‐phosphatidic acid in developing soybeans and soybean suspension cultures
Author(s) -
Stearns E. M.,
Morton W. T.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533633
Subject(s) - phosphatidic acid , phospholipid , chemistry , hydrolysis , chromatography , glycerol , alkaline hydrolysis , lipidology , biochemistry , organic chemistry , clinical chemistry , membrane
A phospholipid which rapidly accumulates radioactivity from [1‐ 14 C] acetate administered to slices of developing soybeans or to suspension cultures of soybean cells was isolated. Its structure was identified by comparison of its properties and degradation products with those of authentic lipid standards using infrared absorption spectrometry, thin layer chromatography, acetolysis, mild alkaline hydrolysis, and determination of molar ratios of phosphorus, glycerol, and acyl moieties. The structure of the phospholipid was found to be bis ‐phosphatidic acid, a phospholipid previously unreported in higher plants.