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Incorporation of [1‐ 14 C] acetate into lipids of soybean cell suspensions
Author(s) -
Wilson A. C.,
Kates M.,
Roche A. I.
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533621
Subject(s) - phospholipid , phosphatidylcholine , phosphatidic acid , oleic acid , linolenic acid , linoleic acid , glycolipid , fatty acid , chemistry , chromatography , biochemistry , incubation , membrane
Suspension cultures of soybean cells incorporated [1‐ 14 C] acetate very rapidly into the fatty acid moieties of phospholipids and glycolipids when incubated at 26 C for up to 22 hr. The most rapidly labeled lipid was 3‐ sn ‐phosphatidylcholine, which contained 58% of the total fatty acid radioactivity after 16 min; more than 75% of this label was found to be in the oleic acid of the phosphatidylcholine. After longer periods of incubation, the proportion of 14 C label decreased exponentially in phosphatidylcholine and increased markedly in an unidentified phospholipid (tentatively, bis ‐phosphatidic acid), di‐ and triacylglycerols, and glycolipids. The proportion of radioactivity in oleic acid also decreased exponentially, accompanied by increases in linoleic acid first and then in linolenic acid. Most of the labeled linolenic acid at 22 hr was found in the unidentified phospholipid, di‐ and triacylglycerols, and the glycolipid fraction.