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The conversion of phosphatidylethanolamine into phosphatidylcholine labeled in the choline group using methyl lodide, 18‐crown‐6 and potassium carbonate
Author(s) -
Patel K. M.,
Morrisett J. D.,
Sparrow J. T.
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533539
Subject(s) - phosphatidylcholine , phosphatidylethanolamine , chemistry , potassium carbonate , choline , potassium , yield (engineering) , phospholipid , benzene , dimethyl carbonate , nuclear chemistry , medicinal chemistry , chromatography , organic chemistry , biochemistry , catalysis , membrane , materials science , metallurgy
A chemical method for the conversion of phosphatidylethanolamine into phosphatidylcholine is described. Methyl iodide in the presence 18‐crown‐6 (1,4,7,10,13,16‐hexaoxacyclooctadecane) and potassium carbonate in benzene is used to alkylate phosphatidylethanolamine in 2.5 hr at 37 C to give an isotopically enriched phosphatidylcholine. The product is purified conventionally and is obtained in 75% yield.