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Behavior of gangliosides on dialysis
Author(s) -
Ghidoni R.,
Sonnino S.,
Tettamanti G.
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533483
Subject(s) - ganglioside , distilled water , chemistry , dialysis , aqueous solution , methanol , chromatography , clinical chemistry , biochemistry , organic chemistry , medicine
Mixed brain gangliosides or individual ganglioside GM 1 were dissolved in one of the following solvents: (a) water, (b) 0.1 M aqueous KC1, or (c) methanol‐aqueous 0.1 M KC1 at concentrations ranging from 10 −8 to 10 −3 M, and were submitted to dialysis against distilled water for up to 4 days. No significant loss of gangliosides on dialysis was observed when gangliosides were dissolved in water or 0.1 M aqueous KC1, but a loss occurred when the ganglioside solution contained methanol; the loss was greater at the lowest ganglioside concentration. However, losses ceased to occur after 1 day when methanol was removed from the dialysis sac.