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The behavior of rat bile phospholipids in the intestine and in incubation media containing pancreatic juice
Author(s) -
Boucrot P.,
Reisser D.,
Clement J.
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533477
Subject(s) - chemistry , palmitic acid , incubation , hydrolysis , linoleic acid , oleic acid , biochemistry , chromatography , phospholipid , fatty acid , membrane
Samples of radioactive bile were collected from rats after intravenous injection of potassium soaps ([9–10 3 H 2 ] or [1 14 C] oleate, [1 14 C] linoleate or [9–10 3 H 2 ] palmitate). These radioactive acids were chosen because it is well established that, in natural phosphatidyl cholines, palmitic acid is located chiefly at the 1 position and linoleic and oleic acids at the 2 position. After incubation of bile with pancreatic juice, the labeling of unchanged biliary phospholipids was higher when native bile was labeled with oleic acid than with palmitic or linoleic acids. These data suggest that monounsaturated molecular species of biliary phospholipids are more resistant than the diunsaturated ones to in vitro hydrolysis by phospholipase A 2 . Ninety min after introduction of the radioactive bile into the upper part of the rat duodenum, high labeling of luminal phospholipids was observed regardless of the bile sample used, although labeling of free fatty acids was always low. The passage of intact biliary phospholipids through the intestinal epithelium is discussed.