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A rapid sensitive method for determining phospholipid phosphorus involving digestion with magnesium nitrate
Author(s) -
DuckChong Coral G.
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533467
Subject(s) - chemistry , reagent , phosphorus , ammonium molybdate , hydrochloric acid , magnesium nitrate , phosphate , magnesium , inorganic chemistry , phospholipid , chromatography , dissolution , malachite green , molybdate , absorbance , digestion (alchemy) , biochemistry , organic chemistry , raw material , adsorption , membrane
A method is described for the rapid determination of phospholipid phosphorus in samples containing less than 0.5 μg phosphorus. Phospholipid phosphorus is first converted to inorganic phosphate by heating a dried lipid extract briefly over a Bunsen flame in the presence of magnesium nitrate, then dissolving the resulting residue in dilute hydrochloric acid at 95 C. The determination of the inorganic phosphate content of the sample then requires only the addition of Triton X‐100 and an acidic ammonium molybdate‐malachite green reagent. Absorbance is measured at 650 nm after 5 min at room temperature.