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The effect of steroids and their solubilizing agents on mycelial growth of Phytophthora cactorum
Author(s) -
Nes W. David,
Patterson Glenn W.,
Bean George A.
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533462
Subject(s) - campesterol , sterol , mycelium , cholestanol , chemistry , phytophthora cactorum , chromatography , cholesterol , biochemistry , botany , biology , phytophthora
Mycelia of Phytophthora cactorum , a fungus unable to synthesize sterols, were incubated with a series of sterols and several common sterol solubilizing agents. Cholesterol, campesterol, and sitosterol promoted growth, while carpesterol, saringosterol, 20 α‐hydroxy cholesterol, coprostanol, and 5α‐cholestane inhibited growth. Cholestanol, epicholestanol, and 5α‐cholestan‐3‐one had no significant effect on growth relative to the sterol‐free control. All steroids were solubilized in ethanol for studying structure‐activity correlations. The added steroids were reisolated from mycelia and their identity confirmed by gas liquid chromatography (GLC) or combined gas liquid chromatography‐mass spectrometry (GLC‐MS). Tween 80 promoted growth, Triton X‐100 inhibited growth, and ethanol had no effect on growth relative to a control.