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Separation of tetra‐, penta‐, and hexaacyl triglycerides by high performance liquid chromatography
Author(s) -
PayneWahl Kathleen,
Plattner Ronald D.,
Spencer Gayland F.,
Kleiman Robert
Publication year - 1979
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533443
Subject(s) - chromatography , chemistry , glyceride , high performance liquid chromatography , degree of unsaturation , acyl group , tetra , thin layer chromatography , elution , silica gel , fatty acid , triglyceride , lipidology , organic chemistry , cholesterol , biochemistry , group (periodic table) , medicinal chemistry
Synthetic triglycerides with more than three acyl groups were prepared by forming estolides from triglycerides containing one, two, or three monohydroxy fatty acyl moieties. These tetra‐, penta‐, and hexaacyl triglycerides were examined by high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). HPLC separation by the number of acyl groups was obtained with both conventional phase and reverse phase columns. In all systems, triglycerides were eluted first, followed in sequence by tetra‐, penta‐, and hexaacyl triglycerides. Within each glyceride class, further separation occurred due to variations in chain length and degree of unsaturation among the component acids. TLC migration on silica gel was found to be a function of the number of acyl groups and the length of component acid chains.