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Blood sampling techniques for studying rapidly turning over metabolic fuels in mice
Author(s) -
Baker Nome,
Morris Daniel,
Sandborg Christy
Publication year - 1976
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533411
Subject(s) - venous blood , clinical chemistry , lipidology , palmitic acid , chemistry , fatty acid , medicine , blood sampling , arterial blood , endocrinology , biochemistry , biology
Experiments were carried out in control and Ehrlich ascites carcinomatous mice to determine whether orbital venous sinus blood could be used to reflect blood in the systemic circulation (decapitation blood) in the case of a rapidly turning over metabolic fuel such as free fatty acids. The early time course of intravenously injected, labeled free fatty acids was measured using [9,10‐ 3 H]palmitic acid and [1‐ 14 C]linoleate complexed to mouse serum. No significant differences between decapitation and orbital sinus blood were found at early times in either group of mice. The orbital sinus clearly contains blood that is not stagnant and is replaced so rapidly that it is suitable for studying very rapidly turning over, circulating metabolites.