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Isotopic labeling of phosphatidylcholine in the choline moiety
Author(s) -
Smith G. A.,
Montecucco C.,
Bennett J. P.
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533373
Subject(s) - phosphatidylcholine , moiety , chemistry , choline , diazomethane , lecithin , ethanolamine , phosphocholine , organic chemistry , chromatography , biochemistry , phospholipid , membrane
Two simple methods for the synthesis of phosphatidylcholine (lecithin) isotopically labeled in the methyl group of the choline moiety are described. Phosphatidylcholine is converted to phosphatidyl‐ethanolamine by enzymic transphosphatidylation, and this is methylated using either methyl iodide or diazomethane to give a product isotopically enriched in all three choline methyl groups.