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Sterol composition of Neurospora crassa
Author(s) -
Renaud R. L.,
Subden R. E.,
Pierce A. M.,
Oehlschlager A. C.
Publication year - 1978
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533367
Subject(s) - sterol , lanosterol , ergosterol , neurospora crassa , chromatography , fraction (chemistry) , lipidology , chemistry , biology , clinical chemistry , biochemistry , cholesterol , mutant , gene
The sterols extracted from freeze‐dried, log‐phase cultures of Neurospora crassa were separated by thin layer and analyzed by capillary gas liquid chromatography and mass spectrometry. Ergosterol, episterol, and ergosta‐7,22 dienol were the major sterols of the free sterol fraction. The major esterified sterols, which constituted 4.95% of the total sterol fraction, were ergosterol, episterol, and ergosta‐ 7,22,24(28)‐trienol, with lesser quantities of 4α‐methyl‐ergosta‐8,24(28)‐dienol. With the exception of lanosterol, all sterols were alkylated at the C‐24 position.