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A simple method for the preparation of pure 9‐ d ‐hydroperoxide of linoleic acid and methyl linoleate based on the positional specificity of lipoxygenase in tomato fruit
Author(s) -
Matthew J. A.,
Chan H. W. S.,
Galliard T.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533358
Subject(s) - linoleic acid , lipoxygenase , yield (engineering) , chemistry , incubation , clinical chemistry , lipidology , organic chemistry , chromatography , biochemistry , fatty acid , enzyme , materials science , metallurgy
Incubation of linoleic acid with crude homogenate of tomato fruit gave a high yield (69%) of linoleic acid hydroperoxides with a ratio of 9‐ to 13‐hydroperoxide isomers of 96∶4. After chromatography of the products, as free acids or methyl esters, hydroperoxides with 9‐ to 13‐isomeric ratios of >99∶1 were obtained. The major product was characterized as 9‐ d ‐hydroperoxy‐octadeca‐ trans ‐10, cis ‐12‐dienoic acid. The results demonstrate the positional specificity of lipoxygenase from tomato fruit.