A simple method for the preparation of pure 9‐ d ‐hydroperoxide of linoleic acid and methyl linoleate based on the positional specificity of lipoxygenase in tomato fruit | Zendy

Matthew J. A. | Zendy; Chan H. W. S. | Zendy; Galliard T. | Zendy

Premium

A simple method for the preparation of pure 9‐ d ‐hydroperoxide of linoleic acid and methyl linoleate based on the positional specificity of lipoxygenase in tomato fruit

Author(s) -

Matthew J. A.,

Chan H. W. S.,

Galliard T.

Publication year - 1977

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02533358

Subject(s) - linoleic acid , lipoxygenase , yield (engineering) , chemistry , incubation , clinical chemistry , lipidology , organic chemistry , chromatography , biochemistry , fatty acid , enzyme , materials science , metallurgy

Incubation of linoleic acid with crude homogenate of tomato fruit gave a high yield (69%) of linoleic acid hydroperoxides with a ratio of 9‐ to 13‐hydroperoxide isomers of 96∶4. After chromatography of the products, as free acids or methyl esters, hydroperoxides with 9‐ to 13‐isomeric ratios of >99∶1 were obtained. The major product was characterized as 9‐ d ‐hydroperoxy‐octadeca‐ trans ‐10, cis ‐12‐dienoic acid. The results demonstrate the positional specificity of lipoxygenase from tomato fruit.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research