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A simplified colorimetric micromethod for determination of serum cholesterol
Author(s) -
Bhandaru Rao R.,
Srinivasan Sathanur R.,
Pargaonkar Padmakar S.,
Berenson Gerald S.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533337
Subject(s) - chromatography , colorimetry , chemistry , reagent , blood serum , cholesterol , biochemistry , medicine , organic chemistry
A simple and rapid micromethod is described for quantitation of cholesterol in 5–10 μl of serum using uranyl acetate to deproteinize the serum and o ‐phthalaldehyde reagent to develop color. Independent analyses of serum samples by the present method and by two reference methods, Abell‐Kendall and automated colorimetric method, indicated accuracy of the micromethod (r=0.99). Use of capillary blood specimens makes this procedure applicable for screening infants and small children.