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Leucine and isoleucine as in vitro precursors for lipid synthesis by rat aorta
Author(s) -
Stillway Lewis W.,
Weigand Dorothy A.,
Riefler John F.,
Buse Maria G.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533327
Subject(s) - lipidology , isoleucine , leucine , in vitro , clinical chemistry , aorta , chemistry , biochemistry , medicine , amino acid
The in vitro conversion of 14 C‐labeled leucine, isoleucine, and pyruvate to specific lipids was compared in rat aorta, diaphragm, and fat pad. Total lipid specific radioactivity from all precursors was greatest in aorta. The ratio of label incorporated into polar lipids vs. neutral lipids by aorta was generally several‐fold that incorporated by muscle and fat pad. The labeling of sterols in the aorta from 14 C‐leucine and pyruvate was equivalent. It is concluded that leucine may be a substantial precursor to polar lipids and to sterols in rat aorta.