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Influence of trichloracetic acid‐phosphotungstic acid on the thin layer chromatographic mobility of gangliosides
Author(s) -
Seyfried T. N.,
Weber E. J.,
Yu R. K.
Publication year - 1977
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02533321
Subject(s) - phosphotungstic acid , chemistry , chromatography , trichloroacetic acid , hydrolysis , dialysis , tungstic acid , salt (chemistry) , ethylenediamine , ultrafiltration (renal) , biochemistry , organic chemistry , medicine , catalysis
Trichloroacetic acid (TCA)‐phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA‐PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA‐PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.