Determination of lipid conjugated dienes with tetracyano‐ethylene‐ 14 C: Significance for study of the pathology of lipid peroxidation | Zendy

Waller Robert L. | Zendy; Recknagel Richard O. | Zendy

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Determination of lipid conjugated dienes with tetracyano‐ethylene‐ 14 C: Significance for study of the pathology of lipid peroxidation

Author(s) -

Waller Robert L.,

Recknagel Richard O.

Publication year - 1977

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02533311

Subject(s) - chemistry , conjugated system , degree of unsaturation , organic chemistry , conjugated diene , diene , lipid peroxidation , tetracyanoethylene , biochemistry , antioxidant , natural rubber , monomer , polymer

A method for quantitative analysis of conjugated diene unsaturation has been developed utilizing tetracyanoethylene‐ 14 C (TCNE‐ 14 C) in a Diels‐Alder condensation. The amount of C 14 found in the Diels‐Alder adduct has been shown to be a measure of conjugated diene content. The method has proven successful in analysis of a variety of triglycerides, phospholipids, and peroxidized tissue lipids. In the course of this work, a method for removing the fatty acid substituents from phospholipids using lithium aluminum hydride was developed. TCNE‐ 14 C analysis for conjugated dienes in rat liver microsomal lipids after dosing with CCl 4 or BrCCl 3 has provided conclusive evidence that the increase in ultraviolet absorption at 233 nm of these lipids is due to conjugated dienes.

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